Background:

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Applications:

WB, IHC

Name of antibody:

GAD1/2

Immunogen:

Synthesized peptide derived from human GAD1/GAD2.

Full name:

glutamate decarboxylase 1 (brain, 67kDa)/2 (pancreatic islets and brain, 65kDa)

Synonyms：

GAD; SCP; CPSQ1/GAD65

SwissProt:

Q05329/Q05329

IHC positive control:

Human lung carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

65 kDa

WB Positive control:

Mouse brain tissue lysate

WB Recommended dilution:

500-3000