Background:

Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha is an enzyme that in humans is encoded by the PIP5K1A gene. Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P.

Applications:

ELISA, WB, IHC

Name of antibody:

PIP5K1A

Immunogen:

Fusion protein of human PIP5K1A

Full name:

phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

SwissProt:

Q99755

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human colon cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

63 kDa

WB Positive control:

Hela, A431 and 293T cells

WB Recommended dilution:

200-1000