Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms.

Applications:

ELISA, WB, IHC

Name of antibody:

MRPL20

Immunogen:

Fusion protein of human MRPL20

Full name:

mitochondrial ribosomal protein L20

Synonyms:

L20mt; MRP-L20

SwissProt:

Q9BYC9

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer and Human brain

IHC Recommend dilution:

50-200

WB Predicted band size:

17 kDa

WB Positive control:

RAW264.7 cell lysate

WB Recommended dilution:

500-2000