Background:

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes.

Applications:

WB, IF

Name of antibody:

PHKA1/2

Immunogen:

Synthesized peptide derived from internal of human KPB1/2.

Full name:

phosphorylase kinase, alpha 1 (muscle)/alpha 2 (liver)

Synonyms：

PHKA/PHK; PYK; XLG; PYKL; XLG2; GSD9A

SwissProt:

P46020/P46019

WB Predicted band size:

137 kDa

WB Positive control:

Jurkat cells and COLO205 lysates

WB Recommended dilution:

500-3000

IF positive control:

Hela cells

IF Recommend dilution:

100-500