Background:

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

Applications:

ELISA, WB, IHC

Name of antibody:

U2AF1

Immunogen:

Fusion protein of human U2AF1

Full name:

U2 small nuclear RNA auxiliary factor 1

Synonyms:

RN; FP793; U2AF35; U2AFBP; RNU2AF1

SwissProt:

Q01081

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer and Human gastric cancer

IHC Recommend dilution:

200-400

WB Predicted band size:

28 kDa

WB Positive control:

231 cell, Rat liver tissue lysates

WB Recommended dilution:

500-2000