Background:

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Applications:

WB, IHC, IF

Name of antibody:

PRKCQ

Immunogen:

Synthetic peptide of human PRKCQ (Ab-676)

Full name:

protein kinase C, theta

Synonyms：

PRKCT; nPKC-theta

SwissProt:

Q04759

IHC positive control:

Human lung carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

82 kDa

WB Positive control:

PC12 cells

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200