Background:

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1

Applications:

WB, IHC, IF

Name of antibody:

MAPK1/MAPK3 (Phospho-Thr185/202)

Immunogen:

Synthetic peptide of human MAPK1/MAPK3 (Phospho-Thr185/202)

Full name:

mitogen-activated protein kinase 1/3 (Phospho-Thr181/202)

Synonyms:

ERK1/2

SwissProt:

P27361/P28482

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

42 kDa; 44 kDa

WB Positive control:

293 cells treated with PMA

WB Recommended dilution:

500-1000

IF positive control:

Hela cells

IF Recommend dilution:

100-200