Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count.

Applications:

ELISA, WB, IHC

Name of antibody:

PSMD3

Immunogen:

Fusion protein of human PSMD3

Full name:

proteasome (prosome, macropain) 26S subunit, non-ATPase, 3

Synonyms：

S3; P58; RPN3; TSTA2

SwissProt:

O43242

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human liver cancer and human ovarian cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

61 kDa

WB Positive control:

293T, Hela and Jurkat cells

WB Recommended dilution:

200-1000