Background:

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Applications:

WB, IHC, IF

Name of antibody:

PAK1 (Phospho-Thr212)

Immunogen:

Synthetic peptide of human PAK1 (Phospho-Thr212)

Full name:

p21 protein (Cdc42/Rac)-activated kinase 1 (Phospho-Thr212)

Synonyms：

PAKalpha

SwissProt:

Q13153

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

68 kDa

WB Positive control:

293 cells untreated or treated with forskolin

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200