Background:

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.

Applications:

WB, IHC

Name of antibody:

PRKAR2B(Ab-113)

Immunogen:

Synthesized non-phosphopeptide derived from human PKA-R2β around the phosphorylation site of serine 113 (R-A-S(p)-V-C).

Full name:

protein kinase, cAMP-dependent, regulatory, type II, beta

Synonyms：

PRKAR2; RII-BETA

SwissProt:

P31323

IHC positive control:

Human colon carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

46 kDa

WB Positive control:

HepG2 cells and K562 cells lysates

WB Recommended dilution:

500-3000